The Effect of Aerobic Training and Cholecalciferol on IGF-I and IGFBP-3 in the Bone Tissue of Rats Poisoned with Hydrogen Peroxide

Background: Exercise and vitamin D can improve bone density by reducing loss. Growth factors such as IGF-I IGFBP-3 are appeared to increase turnover in response mechanical load, free radicals attenuate the release of these growth factors. Objectives: We assessed effect concurrent aerobic training cholecalciferol administration along with hydrogen peroxide injection on levels expression tissue rats. Methods: Thirty-six adult Wistar rats were randomized into six groups (n = 6), including healthy control, sham, (H2O2), H2O2 + training, cholecalciferol, cholecalciferol. The intraperitoneally administered one mmol/kg. Body weight (BW) three times a week even days 0.5 μg/kg.bw daily. Aerobic (at speed 4 - 20 m/min, for 60 minutes) was performed five days/w eight weeks. measured real-time (RT)-PCR. Data analyzed using independent t-test, two-way ANOVA (exercise × D), Bonferroni’s post-hoc test SPSS 26 at significance level P ≤ 0.05. Results: results showed that significantly reduced gene expressions (P 0.001) tissue. Also, exercise augmented 0.008) 0.0001) analysis had greatest < 0.05). In addition, amplifying effects also remarkable 0.1). Conclusions: load created exerted augmenting IGFBP-3. Moreover, interactive significant.